Author/Editor		Jesenek, Dalija; Perutková, Šárka; Kralj-Iglič, Veronika; Kralj, Samo; Iglič, Aleš
Title		Exocytotic fusion pore stability and topological defects in the membrane with orientational degree of ordering
Type		članek
Vol. and No.		Letnik 52, št. 3/4
Publication year		2012
Volume		str. 277-282
ISSN		0143-4160 - Cell calcium
Language		eng
Abstract		Regulated exocytosis is a process that strongly depends on the formation and stability of the fusion pore. It was indicated experimentally and theoretically that narrow and highly curved fusion pore may be stabilized by accumulation of anisotropic membrane components possessing orientational ordering. On the other hand, narrow fusion pore may also undergo repetitive opening and closing, disruption in the so called kiss and run process or become completely opened in the process of full fusion of the vesicle with the membrane. In this paper we attempt to elucidate the subtle interplay between the stabilizing and destabilizing processes in the fusion neck. A possible physical mechanism which may lead to disruption of the stable fusion pore or complete fusion of the vesicle with the membrane is discussed. It is indicated that topologically driven defects of the in-plane orientational membrane ordering in the region of the fusion pore may disrupt the fusion. Alternatively, it may facilitate repetitive opening and closing of the fusion pore or induce full fusion of the vesicle with the target membrane.