| Author/Editor | Mlakar, Jernej; Kocjan, Boštjan; Hošnjak, Lea; Pižem, Jože; Beltram, Matej; Gale, Nina; Drnovšek-Olup, Brigita; Poljak, Mario | |
| Title | Morphological characteristics of conjunctival squamous papillomas in relation to human papillomavirus infection | |
| Type | članek | |
| Vol. and No. | Letnik 99, št. 3 | |
| Publication year | 2015 | |
| Volume | str. 431-436 | |
| ISSN | 0007-1161 - The British journal of ophthalmology | |
| Language | eng | |
| Abstract | Objective To determine the prevalence of a broad spectrum of human papillomavirus (HPV) types in conjunctival papillomas and a possible difference in clinical and histopathological presentation of HPV-positive and HPV-negative papillomas. Methods Formalin-fixed, paraffin-embedded papilloma tissue specimens obtained from 25 patients were analysed using six different PCR-based methods targeting 87 HPV types from four different papillomavirus (PV) genera: alpha-PV, beta-PV, gamma-PV and mi-PV, and in situ hybridisation for HPV-6/HPV-11. Slides were reviewed for pedunculated or sessile growth, the presence of goblet cells, keratinising or non-keratinising epithelium, elastosis, atypia and koilocytes. Results Alpha-PV types HPV-6 and HPV-11 were detected in 19/25 (76%) conjunctival papilloma tissue specimens, 9 (47%) of which were also HPV-6/HPV-11 positive with in situ hybridisation. Six different beta-PV types - HPV-9, HPV-12, HPV-20, HPV-21, HPV-22, HPV-24 - were additionally detected in four cases, all of which were also HPV-6/HPV-11 positive. No gamma-PVs or mi-PVs were found in any of the tested tissues samples. Extralimbal location (p=0.021), presence of goblet cells (p=0.005), non-keratinising squamous epithelium (p=0.005), and absence of elastosis (p=0.005) were associated with the presence of HPV-6/HPV-11. Conclusions We demonstrated that certain clinical and histological features are more frequently associated with HPV infection and that HPV genera other than alpha-PV are most probably not significant factors in conjunctival papilloma occurrence. | |
| Keywords | ophthalmologia human papillomavirus conjunctival squamous cell papilloma infections |