| Author/Editor | Lunar, Maja M.; Mlakar, Jana; Abecasis, Ana B; Vandamme, Anne-Mieke; Tomažič, Janez; Karner, Primož; Vovko, Tomaž; Pečavar, Blaž; Volčanšek, Gabriele; Poljak, Mario | |
| Title | Molekularna epidemiologija okužbe s HIV v Sloveniji | |
| Translated title | Molecular epidemiology of HIV infection in Slovenia | |
| Type | članek | |
| Source | In: Okužbe spolovil in spolno prenosljive bolezni : [zbornik predavanj] Ljubljana : Medicinski razgledi | |
| Vol. and No. | Letnik 53, št. supl. 6 | |
| Publication year | 2014 | |
| Volume | str. 147-152 | |
| Language | slv | |
| Abstract | BACKGROUND. The aim of the present study was to describe the characteristics of HIV epidemic in Slovenia and further explore the evolutionary history of the subtype B epidemic. METHODS. A total of 367 partial HIV-1 pol sequences from Slovenian patients were included, representing 57 % of all patients diagnosed in Slovenia by the end of 2013. We further studied 223 sequences in the analysis of subtype B phylodynamics. RESULTS. Subtype B was the most prevalent subtype, determined in 313 out of 367 (85.3 %) patients. Subtype A and CRF02_AG were most common non-B subtypes, detected in 6.5 % and 2.5 % of patients. Analysis of HIV-1 subtype B transmission chains revealed 8 major clusters (n % 10 patients), 1 group of 4 patients, 2 trios and 12 transmission pairs, thus leaving only 43 (19.3 %) of Slovenian patients without a local epidemiological link. Bayesian analysis (incorporating molecular clock) estimated several introductions of HIV-1 into Slovenia, with most recent common ancestor of the earliest Slovenian cluster dated to the year 1986. We observed a 10-times faster evolutionary rate of Slovenian sequences compared to rate previously described for pol region. CONCLUSIONS. The majority of Slovenian patients had a local transmission link, indicating a closed HIV community. The observed faster evolutionary rate and thus slower epidemic rate suggests that individuals with a long-lasting infection are the driving force of the epidemic. | |
| Keywords | HIV-1 molekularna epidmeiologija filogenetska analiza mutacijska stopnja HIV-1 molecular epidmeiology phylogeny phylodynamics substituation rate |