Author/Editor		Grolla, Ambra A.; Sim, JA; Lim, Dmitry; Rodríguez, José J.; Genazzani, Armando A.; Verkhratsky, Alexei
Title		Amyloid-b and Alzheimer's disease type pathology differentially affects the calcium signalling toolkit in astrocytes from different brain regions
Type		članek
Vol. and No.		Letnik 4
Publication year		2013
ISSN		2041-4889 - Cell death & disease
Language		eng
Abstract		The entorhinal-hippocampal circuit is severely affected in Alzheimer's disease (AD). Here, we demonstrate that amyloid-beta (Abeta) differentially affects primary cultured astrocytes derived from the entorhinal cortex (EC) and from the hippocampus from non-transgenic controls and 3xTg-AD transgenic mice. Exposure to 100 nM of Abeta resulted in increased expression of the metabotropic glutamate receptor type 5 (mGluR5) and its downstream InsP3 receptor type 1 (InsP3R1) in hippocampal but not in EC astrocytes. Amplitudes of Ca(2+) responses to an mGluR5 agonist, DHPG, and to ATP, another metabotropic agonist coupled to InsP3Rs, were significantly increased in Abeta-treated hippocampal but not in EC astrocytes. Previously we demonstrated that senile plaque formation in 3xTg-AD mice triggers astrogliosis in hippocampal but not in EC astrocytes. The different sensitivities of the Ca(2+) signalling toolkit of EC versus hippocampal astrocytes to Abeta may account for the lack of astrogliosis in the EC, which in turn can explain the higher vulnerability of this region to AD.
Keywords		Alzheimer's disease brain regions astrocytes Alzheimerjeva bolezen možganske regije astrociti