Author/Editor		Hojnik, Maja; Gilburd, Boris; Ziporen, Lea; Blank, Miri; Tomer, Yaron; Scheinberg, Morton A; Tincani, Angela; Rozman, Blaž; Shoenfeld, Yehuda
Title		Anticardiolipin antibodies in infections are heterogenous in their dependency on beta2-glycoproetin I: analysis of anticardiolipin antibodies in leprosy
Type		članek
Source		Lupus
Vol. and No.		Letnik 3, št. 6
Publication year		1994
Volume		str. 515-21
Language		eng
Abstract		We studied the effect of beta2-GPI on binding of antibodies in sera from patients with leprosy and patients with the antiphospholipid syndrome (APS) to CL in enzyme-linked immunosorbent assays (ELISAs). Increased levels of IgG aCL were detected in 59 of 61 leprosy patients' sera by the standard aCL-ELISA in the presence of bovine beta2-GPI and in 60 of the 61 leprosy patients' sera by the modified aCL-ELISA without beta2-GPI. When tested by both aCL-ELISAs on the same plate, 10/31 leprosy sera and 9/10 APS sera bound better in the standard aCL-ELISA, 16/31 leprosy sera bound better in the modified aCL-ELISA and in five leprosy and one APS sera the difference was not significant. A dose-dependent enhancing effect of beta2-GPI on the leprosy and APS sera binding to CL was confirmed using purified human beta2-GPI. Enhanced binding was seen if beta2-GPI was added either before or together with the test serum. In 11/61 leprosy sera increased levels of IgG antibodies against beta2-GPI were found by ELSA. Leprosy anti-beta2-GPI antibodies appear to be a separate antibody population recognizing only beta2-GPI adsorbed on the ELISA plate. These results demonstrate heterogeneity of leprosy aCL with respect to their beta2-GPI requirement for binding to CL.
Descriptors		LEPROSY, LEPROMATOUS ANTIBODIES, ANTICARDIOLIPIN ANTIBODIES, ANTIPHOSPHOLIPID ALPHA-MACROGLOBULINS ENZYME-LINKED IMMUNOSORBENT ASSAY