Author/Editor		Poljak, M; Barlič, J; Seme, K; Avšič-Županc, T; Zore, A
Title		Isolation of DNA from archival Papanicolaou stained cytological smears using a simple salting-out procedure
Type		članek
Source		JCP, Clin Mol Pathol
Vol. and No.		Letnik 48
Publication year		1995
Volume		str. M55-M56
Language		eng
Abstract		DNA from archival Papanicolaou stained and unstained cytological smears was sucessfully isolated using a simple, rapid and inexpensive salting-out procedure. The quality of DNA was controlled by polymerase chain reaction (PCR) amplification of segments of the human beta-globin, human beta-actin and human papillomavirus L1 genes. Only negligible differences in amplification efficiency were observed between DNA isolated from stained and unstained smears. The salting-out procedure is a more rapid method for the isolation of DNA than phenolchloroform extraction and may be used in instances where fresh or cryopreserved clinical specimens are not available.
Descriptors		VAGINAL SMEARS DNA PROBES, HPV SPECIMEN HANDLING POLYMERASE CHAIN REACTION