Author/Editor     Pšeničnik, M; Pipan, N
Title     Cytochemical staining of vacuolar compartments in the developing small intestine of mice
Type     članek
Source     Folia histochemica et cytobiologica
Vol. and No.     Letnik 33, št. 1
Publication year     1995
Volume     str. 19-23
Language     eng
Abstract     Osmium impregnation, nicotinamide adenine dinucleotide phosphatase (NADPase) and thiamine pyrophosphatase (TPPase) localisation have been applied as a cytochemical tool for the labelling of the endomembranes in the small intestinal cells of mice during embryonic development. Only cisternae of the endoplasmic reticulum were stained by prolonged osmofication in the undifferentiated cells. At later development stage, Os-black was found in cis Golgi cisternae, small vesicles and also in the endoplasmic reticulum of some epithelial cells. At the early embryonic stage the product of NADPase reaction was visible in medial saccules of the Golgi cimplex and in lysosomes, while at the later stages positive staining appeared also on the apical cell surface and within the structures probably belonging to endosomal compartment. These results indicate that the constantly present in trans Golgi cisternae of the cells during the studied period of fetal life. These results indicate that the cytochemical markers for Golgi staining can also be found in several other endomembrane systems. They give the insight into various connections between the particular intracellular compartments occurring at different development stages.
Descriptors     INTESTINE, SMALL
INTRACELLULAR MEMBRANES
HISTOCYTOCHEMISTRY
MICE
OSMIUM TETROXIDE
THIAMINE PYROPHOSPHATASE
PHOSPHORIC MONOESTER HYDROLASES
NADP