Author/Editor		Kocjan, Boštjan; Hošnjak, Lea; Poljak, Mario
Title		Detection of alpha human papillomaviruses in archivalformalin-fixed, paraffin-embedded (FFPE) tissue specimens
Type		članek
Publication year		2015
ISSN		1386-6532 - Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology
Language		eng
Abstract		Formalin-fixed, paraffin-embedded (FFPE) tissue specimens stored in pathology departments world-wide are an invaluable source for diagnostic purposes when fresh clinical material is unavailable as wellas for retrospective molecular and epidemiological studies, especially when dealing with rare clinicalconditions for which prospective collection is not feasible. Accurate detection of HPV infection in thesespecimens is particularly challenging because nucleic acids are often degraded and therefore, not suitablefor amplification of larger fragments of the viral genome or viral gene transcripts. This review providesa brief summary of molecular methods for detecting alpha-HPV DNA/RNA in FFPE tissue specimens. We specifically address the key procedural and environmental factors that have the greatest impact onthe quality of nucleic acids extracted from FFPE tissue specimens, and describe some solutions that canbe used to increase their integrity and/or amplifiability. Moreover, commonly used methods for HPVDNA/RNA detection in FFPE tissue specimens are presented and discussed, focusing on studies usingpolymerase chain reaction as an HPV detection method and published after 1999. Finally, we brieflysummarize our 22 years of experience with HPV detection in FFPE tissue specimens.
Keywords		Human papillomaviruses formalin stored tissue samples človeški papilomavirusi formalin shranjeni vzorci tkiv