Author/Editor		Klemenak, Martina; Dolinšek, Jernej; Langerholc, Tomaž; Di Gioia, Diana; Mičetić-Turk, Dušanka
Title		Administration of Bifidobacterium breve decreases the production of TNF-[alfa] in children with celiac disease
Type		članek
Vol. and No.		Letnik 60, št. 11
Publication year		2015
Volume		str. 3386-3392
ISSN		0163-2116 - Digestive diseases and sciences
Language		eng
Abstract		BACKGROUND: Increasing evidence suggests that not only genetics, but also environmental factors like gut microbiota dysbiosis play an important role in the pathogenesis of celiac disease (CD). AIM: The aim of our study was to investigate the effect of two probiotic strains Bifidobacterium breve BR03 and B. breve B632 on serum production of anti-inflammatory cytokine interleukin 10 (IL-10) and pro-inflammatory cytokine tumor necrosis factor alpha (TNF-[alfa]) in children with CD. METHODS: The study was a double-blinded, placebo-controlled trial that included 49 children with CD on gluten-free diet (GFD) randomized into two groups and 18 healthy children in the control group. The first group (24 children with CD) daily received B. breve BR03 and B632 (2 % 109 colony-forming units) and the second group (25 children with CD) received placebo for 3 months. RESULTS: TNF-[alfa] levels were significantly decreased in the first group after receiving B. breve for 3 months. On follow-up, 3 months after receiving probiotics, TNF-[alfa] levels increased again. Children with CD who were on GFD for less than 1 year showed similar baseline TNF-[alfa] levels as children who were on GFD for more than 1 year. IL-10 levels were in all groups of patients below detection level. CONCLUSIONS: Probiotic intervention with B. breve strains has shown a positive effect on decreasing the production of pro-inflammatory cytokine TNF-[alfa] in children with CD on GFD.
Descriptors		Celiac Disease
Keywords		Bifidobacterium breve Cytokines Children