| Author/Editor | Žigon, Polona; Božič, Mojca; Frank, Mojca; Ambrožič, Aleš; Tomšič, Matija; Hočevar, Alojzija; Božič, Borut; Sodin-Šemrl, Snežna; Kveder, Tanja; Čučnik, Saša | |
| Title | Laboratory methodology important in the diagnosis and prognosis of antiphospholipid syndrome | |
| Type | članek | |
| Source | In: Thrombosis, atherosclerosis and atherothrombosis - new insights and experimental protocols Rijeka : InTech | |
| Publication year | 2015 | |
| Volume | str. 105-141 | |
| Language | eng | |
| Abstract | Antiphospholipid syndrome (APS) is an autoimmune disease, characterized by thrombosis and pregnancy complications with persistently elevated levels of antiphospholipid antibodies (aPL). Recently,a unique mathematical calculation has been presented to assess the risk of thrombosis in patients with APS called antiphospholipid score or global antiphospholipid syndrome score (GAPSS). This new approach in thediagnosis of APS leads to the assessment of the risk of thrombosis considering the results of different aPL (lupus anticoagulants (LA), anticardiolipin antibodies (aCL), antibodies against ß 2GPI (anti- ß 2GPI), and phosphatidylserine- dependent antiprothrombin antibodies (aPS/PT)(isotypes IgG and IgM). This chapter provides an overview of the algorithm strategy for APS diagnosis with the aims of characterizing in detail the laboratory methodology of criteria aPL (LA, aCL, and anti- ß 2GPI) and noncriteria aPL, such as IgA aCL and IgA anti- ß 2GPI, anti-domain I ß 2GPI, and antiprothrombin antibodies. In order to improve APS diagnosis, several new approaches in aPL detection have recently been suggested, such as multiline immunodot assay, detection of aPL by flow cytometry using beads with particular surface properties, and the newly developed automated BioPlex system technology for parallel detection of aCL and anti- ß 2GPI antibodies of IgG, IgA, and IgM isotypes. A completely different and promising approach in future research lies in the potential of microRNAs as biomarkers for risk of thrombosis and/or obstetric complication. | |
| Descriptors | Imunologija | |
| Keywords | anticardiolipin antibodies lupus anticoagulants anti-beta2-glycoprotein I antibodies antiprothrombin antibodies ELISA multiplex assays microRNA studies |