Author/Editor		Chowdhury Haque, Helena; Velebit Marković, Jelena; Radić, Nataša; Frančič, Vito; Kreft, Marko; Zorec, Robert
Title		Hypoxia alters the expression of dipeptidyl peptidase 4 and induces developmental remodeling of human preadipocytes
Type		članek
Vol. and No.		, št. Vol. 2016
Publication year		2016
Volume		str. 1-9
ISSN		2314-6753 - Journal of diabetes research
Language		eng
Abstract		Dipeptidyl peptidase 4 (DPP4), a transmembrane protein, has been identified in human adipose tissue and is considered to be associated with obesity-related type 2 diabetes. Since adipose tissue is relatively hypoxic in obese participants, we investigated the expression of DPP4 in human preadipocytes (hPA) and adipocytes in hypoxia, during differentiation and upon insulin stimulation. The results show that DPP4 is abundantly expressed in hPA but very sparsely in adipocytes. During differentiation in vitro, the expression of DPP4 in hPA is reduced on the addition of differentiation medium, indicating that this protein can be hPA marker. Long term hypoxia altered the expression of DPP4 in hPA. In in vitro hypoxic conditions the protease activity of shed DPP4 is reduced; however, in the presence of insulin, the increase in DPP4 expression is potentiated by hypoxia.
Keywords		transmembrane protein hypoxia type 2 diabetes transmembranski protein hipoksija diabetes tip 2