| Author/Editor | Vlaisavljević, Veljko; Križančić Bombek, Lidija; Kokalj-Vokač, Nadja; Kovačič, Borut; Čižek-Sajko, Mojca | |
| Title | How safe is germinal vesicle stage oocyte rescue? Aneuploidy analysis of in vitro matured oocytes | |
| Type | članek | |
| Vol. and No. | Letnik 134, št. 2 | |
| Publication year | 2007 | |
| Volume | str. 213-219 | |
| ISSN | 1872-7654 - European journal of obstetrics, gynecology, and reproductive biology | |
| Language | eng | |
| Abstract | Objective: To evaluate the rate and type of aneuploidies of chromosomes 13, 16, 18, 21 and 22, with respect to the length of in vitro maturation (IVM) period, and to compare the results to previously published studies on aneuploidy rates of unfertilized, uninseminated mature oocytes and first polar bodies. Study design: Two hundred and twelve immature germinal vesicle stage oocytes were assigned to two groups. After successful IVM, depending on their maturational period of 24h (Group A) or 36h (Group B), chromosomal analysis was performed by five color fluorescence in situ hybridization (FISH). In Groups A and B the rates of aneuploid oocytes were calculated and compared by chi-square test. Also the rates of hyperhaploidy, hypohaploidy, disomy and nullisomy were determined and compared by chi-square test. The difference was considered statistically significant at p-value of <0.05. Results: The prolonged IVM did not significantly affect the aneuploidy rate compared to the shorter maturation period (48.1% and 45.0%, respectively). Regarding the unbalanced premature chromatid separation, no statistically significant difference was found between hyperhaploidy and hypohaploidy (14.8% versus 8.3%). For chromosome nondisjunction, higher frequency of disomy than nullisomy was observed (30.6% versus 14.8%; p<0.05). The estimated global aneuploidy rate was between 42% and 63%. Conclusins: The aneuploidy rate of IVM GV-oocytes is comparable to the aneuploidy rate of in vivo matured oocytes and first polar bodies, regardless of the length of maturation period. This suggests that the immature oocytes can be used in infertility treatment after they complete maturation. | |
| Descriptors | Infertility Neplodnost Fertilization in Vitro Oploditev in vitro Reproductive Techniques, Assisted Reproduktivne tehnike, asistirane Embryonic Development Embrionalni razvoj Aneuploidy Anevploidija Oocytes Oociti Chromosomes Kromosomi In Situ Hybridization, Fluorescence In situ hibridizacija, fluorescenčna |