Author/Editor		Jenko, Barbara; Praprotnik, Sonja; Tomšič, Matija; Dolžan, Vita
Title		NLRP3 and CARD8 polymorphisms influence higher disease activity in rheumatoid arthritis
Translated title		Polimorfizmi NLRP3 I CARD8 utiču na pojačanu aktivnost bolesti u reumatoidnom artritisu
Type		članek
Publication year		2016
Language		eng
Abstract		Background: The activation of NLRP3-inflammasome may contribute to inflammatory processes in rheumatoid arthri- tis (RA). Functional polymorphisms in the genes coding for its components NLRP3 and CARD8 were associated with a proinflammatory phenotype. Our aim was to investigate the influence of these polymorphisms on RA susceptibility and disease activity at the time of diagnosis and after six months of treatment. Methods: A group of 128 RA patients treated with metho - trexate and 122 healthy controls were genotyped for NLRP3 rs35829419 (p. Q705K) and CARD8 rs2043211 (p. C10X) polymorphisms. Results: RA susceptibility was not influenced by the investi- gated polymorphisms or their interaction. The investigated polymorphisms explained 8% of variability in DAS28 at the time of diagnosis. Carriers of NLRP3 rs35829419 or CARD8 rs2043211 polymorphisms had significantly high- er DAS28 at the time of diagnosis (p=0.003; p=0.022; respectively). Polymorphic CARD8 rs2043211 TT geno- type was also associated with higher DAS28 after six months of treatment (p=0.033). Conclusions: Genetic variability of inflammasome compo- nents may contribute to higher disease activity at the time of diagnosis and after 6 months of methotrexate treatment in RA patients. Better understanding of the immunological mechanisms behind a more active course of RA may sug- gest novel treatment approaches in a subset of patients with a proinflammatory phenotype.
Keywords		polymorphism rheumatoid arthritis inflammasome polimorfizem reumatoisni artritis inflamazom