Author/Editor		Prezelj, Marija; Knap, Bojan
Title		Automated assay for non-transferrin-bound iron in serum samples
Type		članek
Vol. and No.		Letnik 48, št. 10
Publication year		2010
Volume		str. 1427-1432
ISSN		1434-6621 - Clinical chemistry and laboratory medicine : CCLM / FESCC
Language		eng
Abstract		Background: Non-transferrin-bound iron (NTBI) is a powerful promoter of free radical damage and highly toxic to biological systems, resulting in oxidative damage to proteins, lipids and DNA. Methods: This assay is based on the binding of serum NTBI by the chelator nitrilotriacetic acid (NTA) and measurement of the ultrafiltrated Fe-NTA complex with the ferrozine reagent kit by a biochemical analyzer. To determine NTBI at extremely low concentrations, the program parameters for serum iron measurement were modified. Results: Linearity was up to 15 mmol/L with analytical recovery of 93%-103%. The limit of detection was 0.076 mmol/L. The within-run coefficient of variation was 2.37%, 1.23%, and 0.812% at concentrations of 0.338, 1.717, and 5.916 mmol/L, respectively. NTBI concentrations measured after exercise insamples obtained from 14 rowers, divided into two groups, were substantiallyhigher in all samples. The median NTBI concentrations (range) before and after exercise were 0.197 (-0.11 to 0.58), and 3.353 (2.39-8.97) mmol/L, respectively, in older rowers and 0.197 (-0.18 to 1.17), and 1.360 (0.47-2.49) mmol/L, respectively, in younger rowers. Conclusions: With the described modification for serum iron determination, NTBI can be measured with high sensitivity and specificity. The data presented are illustrative examples of the applicability of this assay.
Keywords		oksidativni stres NTBI metode