Author/Editor     Barlič-Maganja, D; Štrukelj, B; Pungerčar, J; Gubenšek, F; Turk, V; Kregar, I
Title     Isolation and sequence analysis of the genomic DNA fragment encoding an aspartic proteinase inhibitor homologue from potato (Solanum tuberosum L.)
Type     članek
Source     Plant Mol Biol
Vol. and No.     Letnik 20, št. 2
Publication year     1992
Volume     str. 311-3
Language     eng
Abstract     A genomic DNA clone encoding an aspartic proteinase inhibitor of potato was isolated from a lambda EMBL3 phage library using the aspartic proteinase inhibitor cDNA as a hybridization probe. The gene has all characteristic sequences normally found in eucaryotic genes. Typical CAAT and TATA box sequences were found in the 5'-upstream region. In this part are also two putative regulatory AGGA box sequences located. In the genomic sequence there are no intron sequences interrupting the coding region. An open reading frame of the gene encodes a precursor protein of 217 amino acids which shows high percent identity with the aspartic proteinase inhibitor cDNA.
Descriptors     CATHEPSIN D
POTATOES
PROTEINS
AMINO ACID SEQUENCE
BASE SEQUENCE
BLOTTING, SOUTHERN
DNA
MOLECULAR SEQUENCE DATA
POTATOES
SEQUENCE HOMOLOGY, AMINO ACID