Author/Editor		Križaj, I; Bieber, AL; Ritonja, A; Gubenšek, F
Title		The primary structure of ammodytin L, a myotoxic phospholipase A2 homologue from Vipera ammodytes venom
Type		članek
Source		Eur J Biochem
Vol. and No.		Letnik 202, št. 3
Publication year		1991
Volume		str. 1165-8
Language		eng
Abstract		A new myotoxic phospholipase A2 homologue, having a serine residue in position 49 instead of highly conserved aspartic acid, was found in the venom of Vipera ammodytes. The primary structure revealed additional mutations in the positions important for enzymatic activity. Tyr28 is exchanged for a histidine and Gly33 for asparagine. These changes render earlier-reported weak enzymatic activity unlikely. The role of this rather abundant venom fraction is apparently in myotoxicity, which was confirmed in the muscle-cell culture from neonatal rats. The muscle-cell culture proved to be a good tool to investigate the effects of various myotoxins on muscle cells.
Descriptors		PHOSPHOLIPASES A VIPER VENOMS AMINO ACID SEQUENCE ANIMALS, NEWBORN CELL FUSION CELLS, CULTURED MOLECULAR SEQUENCE DATA MUSCLES RATS SEQUENCE HOMOLOGY, NUCLEIC ACID VIPER VENOMS