Author/Editor		Černe, R; Rusin, KI; Randić, M
Title		Enhancement of the N-methyl-D-aspartate response in spinal dorsal horn neurons by cAMP-dependent protein kinase
Type		članek
Source		Neurosci Lett
Vol. and No.		Letnik 161, št. 2
Publication year		1993
Volume		str. 124-8
Language		eng
Abstract		Glutamate-gated ion channels mediate excitatory synaptic transmission in the central nervous system and are involved in synaptic plasticity, neuronal development and excitotoxicity (5,24). These ionotropic glutamate receptors were classified according to their preferred agonists as AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid), KA (kainate), and NMDA (N-methyl-D-aspartate) receptors ŠTrends Pharmacol. Sci., 11 (1990) 25-33Ć. The present study of NMDA receptor channels expressed in acutely isolated spinal dorsal horn (DH) neurons of young rat reveals that they are subject to modulation through the adenylate cyclase cascade. Whole-cell voltage-clamp recording mode was used to examine the effect of adenosine 3',5'-monophosphate (cAMP)-dependent protein kinase (PKA) on the responses of DH neurons to NMDA. Whole-cell current response to NMDA was enhanced by 8 Br-cAMP, a membrane permeant analog of cAMP or by intracellular application of cAMP or catalytic subunit of PKA.
Descriptors		CYCLIC AMP-DEPENDENT PROTEIN KINASES NEURONS RECEPTORS, N-METHYL-D-ASPARTATE SPINAL CORD ADENOSINE TRIPHOSPHATE AMINO ACIDS CYCLIC AMP ION CHANNEL GATING N-METHYLASPARTATE RATS RATS, SPRAGUE-DAWLEY RECEPTORS, CHOLINERGIC SPINAL CORD BROMO CYCLIC ADENOSINE MONOPHOSPHATE