Author/Editor		Ritonja, A; Machleidt, W; Turk, V; Gubenšek, F
Title		Amino-acid sequence of ammodytoxin B partially reveals the location of the site of toxicity of ammodytoxins
Type		članek
Source		Biol Chem Hoppe Seyler
Vol. and No.		Letnik 367, št. 9
Publication year		1986
Volume		str. 919-23
Language		eng
Abstract		The complete amino-acid sequence of ammodytoxin B, a presynaptically toxic phospholipase A2 isolated from Vipera ammodytes ammodytes venom, was determined by manual and automated protein sequencing. Ammodytoxin B (i.v. LD50 = 0.58 mg/kg for white mice) is 30-fold less toxic than ammodytoxin A, the most toxic phospholipase isolated from the same venom. The two proteins (each 122 residues long) differ in only 3 residues located in positions 115, 118 and 119 (numbering according to R. Renetseder et al. (1985) J. Biol. Chem. 260, 11627-11634) suggesting that an exposed hydrophobic residue in position 115 and a basic residue in position 118 may be responsible for the increased toxicity of ammodytoxin A and should form at least one part of the site of toxicity in ammodytoxins.
Descriptors		PHOSPHOLIPASES PHOSPHOLIPASES A VIPER VENOMS AMINO ACID SEQUENCE CATTLE CYANOGEN BROMIDE MICE PEPTIDE FRAGMENTS STRUCTURE-ACTIVITY RELATIONSHIP VIPER VENOMS