Author/Editor		Pariš, A; Štrukelj, B; Pungerčar, J; Renko, M; Dolenc, I; Turk, V
Title		Molecular cloning and sequence analysis of human preprocathepsin C
Type		članek
Source		FEBS Lett
Vol. and No.		Letnik 369
Publication year		1995
Volume		str. 326-30
Language		eng
Abstract		A cDNA clone (C1) coding for human preprocathepsin C was isolated from a human ileum cDNA library using a rat kidney-derived RT-PCR probe and its complete nucleotide sequence determined. The full-lenght 1857 bp sequence codes for a protein of 463 amino acid residues with a calculated molecular mass of 51848 Da. Comparison of the deduced amino acid sequence with that of rat preprocathepsin C indicates an 87.5 percent identity. A multiple alignemnt of the deduced cathepsin C sequence of 233 residues which, by analogy to other cysteine proteinases, coresponds to the mature protein, confirms that human cathepsin C belongs to the papain superfamily.
Descriptors		CYSTEINE PROTEINASES AMINO ACID SEQUENCE CLONING, MOLECULAR ENZYME PRECURSORS RATS KIDNEY BLOTTING, SOUTHERN CHROMATOGRAPHY, GEL ILEUM