Author/Editor		Milačič, R; Štupar, J
Title		Simultaneous determination of chromium(III) complexes and chromium(VI) by fast protein anion-exchange liquid chromatography- atomic absorption spectrometry
Type		članek
Source		Analyst
Vol. and No.		Letnik 119
Publication year		1994
Volume		str. 627-32
Language		eng
Abstract		Chromium(III) complexes Šoxalate and ethylenediamine-tetraacetic acid (EDTA)Ć and CrVI were separated simultaneously on a fast protein liquid chromatography (FPLC) anion-exchange column of Mono Q HR 5/5. For separation tris(hydroxymethyl)aminomethane (TRIS)-HCI buffer (5 * 10-3 mol dm-3, pH 5.58.5), and the same buffer with NaCl (0.5 mol dm-3), were employed in gradient elution (15 min; flow rate, 1 cm3 min-1). The column was regenerated (NaCl) and equilibrated (TRIS-HCI buffer) in the following 8 min. With this procedure, CrIII-citrate passed through the column, whereas relatively stable anionic CrIII complexes (with EDTA and oxalate), and CrVI were separated and determined by ultraviolet molecular (234 and 273 nm, respectively) and atomic (357.9 nm) absorption. Atomic absorption was measured 'offline' in 100 or 200 micro l eluate fractions. The method was successfullly employed for the determination of CrVI, CrIII-EDTA, and CrIII-oxalate in cabbage xylem at ng cm-3 levels Šlimits of detection (LOD), 5, 3, and 80 ng cm-3, respectivelyĆ, using a 500 micro l sample loop. In addition, chromate was monitored in CrVI containing nutrient solutions during plant experiments to investigate Cr uptake by plants (LOD, 5 ng cm-3)
Descriptors		CHROMIUM COMPOUNDS ENVIRONMENTAL POLLUTANTS SPECTROPHOTOMETRY, ATOMIC ABSORPTION CHROMATOGRAPHY, HIGH PRESSURE LIQUID