Author/Editor		Potočar, Urška; Hudoklin, Samo; Erdani-Kreft, Mateja; Završnik, Janja; Božikov, Krešimir; Fröhlich, Mirjam
Title		Adipose-derived stem cells respond to increased osmolarities
Type		članek
Vol. and No.		Letnik 11, št. 10
Publication year		2016
Volume		str. 1-18
ISSN		1932-6203 - PloS one
Language		eng
Abstract		Cell therapies present a feasible option for the treatment of degenerated cartilaginous and intervertebral disc (IVD) tissues. Microenvironments of these tissues are specific and often differ from the microenvironme nt of cells that, could be potentially used for therapy, e.g. human adipose-derived stem cells (hASC). To ensure safe and efficient implantation of hASC, it is important to evaluate how microenvironme ntal conditions at the site of implanta- tion affect the implanted cells. This study has demonstrated that cartilaginous tissue-spe- cific osmolarities ranging from 400-600 mOsm/L affected hASC in a dose- and time- dependent fashion in comparison to 300 mOsm/L. Increased osmolarities resulted in tran- sient (nuclear DNA and actin reorganisation) and non-transient, long-term morphological changes (vesicle formation, increase in cell area, and culture morphology), as well as reduced proliferation in monolayer cultures. Increased osmolarities diminished acid proteo- glycan production and compactness of chondrogenically induced pellet cultures, indicating decreased chondrogenic potential. Viability of hASC was strongly dependent on the type of culture, with hASC in monolayer culture being more tolerant to increased osmolarity com- pared to hASC in suspension, alginate-agarose hydrogel, and pellet cultures, thus empha- sizing the importance of choosing relevant in vitro conditions according to the specifics of clinical application.
Keywords		stem cell osmolarities cell therapies matične celice osmolarnost celične terapije