Author/Editor | Vukelić, B; Ritonja, A; Renko, M; Pokorny, M; Vitale, L | |
Title | Extracellular alpha-amylase from Streptomyces rimosus | |
Type | članek | |
Source | Applied microbiology and biotechnology | |
Vol. and No. | Letnik 37 | |
Publication year | 1992 | |
Volume | str. 202-4 | |
Language | eng | |
Abstract | A purification procedure for an extracellular alfa-amylase from streptomyces rimosus, oxytetracycline-producing strain, is described. The enzyme obtained was shown to be an acidic (pI 4.75) monomer with a relative molecular mass (Mr) of 43000, containing three cysteines involved in the catalytic activity of the enzyme. Its amino-terminal part has 57-67 percent homology with amylases from other Streptomyces species. S. rimosus alfa-amylase is sensitive to higher temperatures, and partially stabilized by Ca2+ ions. It hydrolyses starch (optimum at pH 5.0-6.0) in an endohydrolase manner giving rise to maltotriose, maltoteatrose and higher oligosaccharides. Starch grannules, except those from rice, were not significantly affected by the isolated alfa-amylase. | |
Descriptors | STREPTOMYCES ALPHA-AMYLASE SPECTROPHOTOMETRY CHROMATOGRAPHY, GEL SEQUENCE ANALYSIS |