Author/Editor		Zemljič Jokhadar, Špela; Božič, Bojan; Kristanc, Luka; Gomišček, Gregor
Title		Osmotic effects induced by pore-forming agent nystatin
Type		članek
Vol. and No.		Letnik 11, št. 10
Publication year		2016
Volume		str. 1-23
ISSN		1932-6203 - PloS one
Language		eng
Abstract		The responses of Chinese hamster ovary epithelial cells, caused by the pore-forming agent nystatin, were investigated using brightfield and fluorescence microscopy. Different phenomena, i.e., the detachment of cells, the formation of blebs, the occurrence of cell-vesicles and cell ruptures, were observed. These phenomena were compared to those discovered in giant lipid vesicles. A theoretical model, based on the osmotic effects that occur due to the size-discriminating nystatin transmembrane pores in lipid vesicles, was extended with a term that considers the conservation of the electric charge density in order to describe the cell's behavior. The increase of the cellular volume was predicted and correlated with the observed phenomena.
Keywords		epithelial cells nystatin lipid vesicles epitelne celice nistatin lipidni vezikli