Author/Editor		Kos, J; Dolinar, M; Turk, V
Title		Isolation and characterisation of chicken L- and H-kininogens and their interaction with chicken cysteine proteinases and papain
Type		članek
Source		Agents Actions Suppl
Vol. and No.		Letnik 38, št. 1
Publication year		1992
Volume		str. 331-9
Language		eng
Abstract		We have isolated L- and H-kininogens from chicken egg white and plasma using affinity chromatography on CM-papain Sepharose, Con-A Sepharose, gel filtration and ion exchange chromatography, in pure form. In chicken plasma mostly HK was present, whereas in chicken egg white both LK and HK were identified. The determined Mr of LK and HK were 69000 and about 96000, respectively. They occurred in multiple forms with pI 4.3-5.2. The inhibitory activity of chicken HK was tested against cysteine proteinases cathepsins B and L, isolated from chicken liver, and papain. The obtained Ki values demonstrated that chicken H-kininogen is a strong inhibitor of chicken cathepsin L and papain, but much weaker inhibitor of chicken cathepsin B.
Descriptors		CHICKENS KININOGENS AMINO ACID SEQUENCE CATHEPSIN B CATHEPSINS CHICK EMBRYO CHROMATOGRAPHY CYSTEINE PROTEINASE INHIBITORS EGG PROTEINS KINETICS KININOGENS MOLECULAR SEQUENCE DATA MOLECULAR WEIGHT PAPAIN