Author/Editor		Bevec, T; Dolenc, I; Ritonja, A; Turk, V
Title		Purification of the complex of cathepsin L and the MHC class II-associated invariant chain fragment from human kidney
Type		članek
Source		FEBS Lett
Vol. and No.		Letnik 336, št. 3
Publication year		1993
Volume		str. 555-9
Language		eng
Abstract		The complex of cathepsin L and the fragment of the MHC class II-associated invariant chain was purified from human kidney. M(r) of the complex, as determined by gel filtration, is about 40,000. Both components were identified by amino acid and sequence analyses. The bound invariant chain fragment is almost identical to the additional segment found in p41, but not in the p31 form of the invariant chain. The complex has significantly enhanced stability at neutral and slightly alkaline pH, and reduced proteolytic activity against the synthetic substrate Z-Phe-Arg-MCA compared to free cathepsin L. The complex exhibits no enzymatic activity against the protein substrate azocasein. For the first time, the invariant chain was found in a complex with a protein, which was not an MHC molecule.
Descriptors		CATHEPSINS HLA-D ANTIGENS KIDNEY BINDING SITES CATHEPSINS CHROMATOGRAPHY, GEL CHROMATOGRAPHY, HIGH PRESSURE LIQUID ELECTROPHORESIS, POLYACRYLAMIDE GEL HLA-D ANTIGENS ISOELECTRIC FOCUSING KINETICS MACROMOLECULAR SYSTEMS MOLECULAR SEQUENCE DATA MOLECULAR WEIGHT PEPTIDE FRAGMENTS PROTEIN BINDING SPECTROMETRY, FLUORESCENCE AMINO ACID SEQUENCE