Author/Editor     Benčina, D; Bradbury, JM
Title     Combination of immunofluorescence and immunoperoxidase techniques for serotyping mixtures of Mycoplasma species
Type     članek
Source     J Clin Microbiol
Vol. and No.     Letnik 30, št. 2
Publication year     1992
Volume     str. 407-10
Language     eng
Abstract     We describe a method for the simultaneous identification of up to three Mycoplasma species by the use of contrast-labeled fluorescent antibodies against two species and peroxidase-labeled antibody against a third species. The procedure enabled the rapid identification of colonies of three artificially mixed avian Mycoplasma species on agar blocks and also mixtures of species in cultures from naturally infected chickens. Furthermore, it was possible to quantitate the components of a mixture of Mycoplasma gallisepticum, M. synoviae, and M. gallinarum. This new procedure offers an improvement over existing methods in terms of both speed and analytical capability.
Descriptors     MYCOPLASMA
SEROTYPING
ANTIBODIES, BACTERIAL
CHICKENS
EVALUATION STUDIES
FLUORESCENT ANTIBODY TECHNIQUE
IMMUNOENZYME TECHNIQUES
MYCOPLASMA
SPECIES SPECIFICITY