Author/Editor		Bedene, Ajda; Mencej Bedrač, Simona; Ješe, Lea; Marc, Janja; Vrtačnik, Peter; Preželj, Janez; Kocjan, Tomaž; Kranjc, Tilen; Ostanek, Barbara
Title		MiR-148a the epigenetic regulator of bone homeostasis is increased in plasma of osteoporotic postmenopausal women
Type		članek
Vol. and No.		Letnik 128, št. suppl. 7
Publication year		2016
Volume		str. 519-526
ISSN		0043-5325 - Wiener klinische Wochenschrift
Language		eng
Abstract		Background: Osteoporosis is a prevalent skeletal disorder characterized by reduced bone mineral density and microarchitectural deterioration of bone tissue, resulting in bone fragility and low trauma fractures. Imaging techniques are used routinely to detect low bone mass; however, they are unable to identify deterioration of bone quality. Recently, microRNAs have emerged as regulators of bone remodelling and potentially also as a new class of sensitive biomarkers of bone health to aid in diagnosis and treatment monitoring of osteoporosis. Methods: To identify new plasma-based biomarkers associated with osteoporosis we analysed microRNAs isolated from plasma samples of 74 postmenopausal women divided into osteoporotic (N=17) and control groups (N=57). A prior microRNA screening was performed where a few showed promise for further analysis. Quantitative polymerase chain reaction was used to investigate differences in expression of let-7d-5p, let-7e-5p, miR-30d-5p, miR-30e-5p, miR-126-3p, miR-148a-3p, miR-199a-3p, miR-423-5p and miR-574-5p, between the two groups. Furthermore, correlation analysis between miRNA expression levels and patient bone mineral density measurements and FRAX as well as trabecular bone scores were performed. Results: Expression of miR-148a-3p was significantly higher (p = 0.042) in the osteoporotic patient group compared to the control. In addition, we identified a correlation between miR-126-3p ([ro] = 0.253, p = 0.032) and 423-5p ([ro] = 0.230, p = 0.049) and parameters of bone quality and quantity. Conclusion: Results from our study, together with the functional role of miR-148a-3p in bone suggest, that this miRNA could be considered as a potential new plasma-based biomarker for pathological changes associated with osteoporosis.
Keywords		circulating microRNA osteoporosis bone mineral density FRAX TBS