Author/Editor		Završnik, Matej; Kariž, Stojan; Makuc, Jana; Šeruga, Maja; Cilenšek, Ines; Petrovič, Danijel
Title		PECAM-1 Leu125Val (rs688) polymorphism and diabetic nephropathy in caucasians with type 2 diabetes mellitus
Type		članek
Vol. and No.		, št. Vol. 2016
Publication year		2016
Volume		str. 1-7
ISSN		2210-7177 - Analytical cellular pathology (Amsterdam)
Language		eng
Abstract		Objectives. Platelet endothelial cell adhesion molecule-1 (PECAM-1) plays a key role in the transendothelial migration of circulating leukocytes during inflammation and in the maintenance of vascular endothelial integrity. We hypothesized that genetic variation in PECAM-1 gene could be associated with diabetic nephropathy (DN) and with the level of soluble PECAM-1 in Caucasians with type 2 diabetes mellitus (T2DM). Design and Methods. We analyzed the rs688 single nucleotide polymorphism of PECAM-1 gene C373G (Leu125Val) at exon 3, which encodes the first extracellular Ig-like domain that mediates the homophilic binding of PECAM-1, in 276 T2DM subjects with documented DN (cases) and 375 T2DM subjects without DN (controls), using a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) strategy. Level of plasma soluble PECAM-1 (sPECAM-1) was measured by ELISA in a subpopulation of 120 diabetics with DN. Results. We found no association between the Leu125Val polymorphism and DN in subjects with T2DM. Likewise, the Leu125Val polymorphism was not associated with serum sPECAM-1 levels in a subpopulation of 120 diabetics with DN. Conclusion. The Leu125Val polymorphism of PECAM-1 and the level of sPECAM-1 are not associated with DN in T2DM subjects of Slovenian origin.
Keywords		diabetes diabetična nefropatija PECAM-1 rs688 diabetes diabetic nephropathy PECAM-1 rs688