Author/Editor		Kveder, Tanja; Lakota, Katja; Švec, Tinka; Čučnik, Saša; Žigon, Polona; Ambrožič, Aleš; Sodin-Šemrl, Snežna; Božič, Borut; Tomšič, Matija
Title		Fluorometric immunoassay for detection of anti-dsDNA antibodies
Translated title		Dosage immunologique fluorométrique pour la détection d'anticorps anti-adndb
Type		monografija
Place		Geneva
Publisher		World Intellectual Property Indicators
Publication year		2015
Language		eng
Abstract		The present invention relates to the field of immunological methods, more precisely to the field of detection methods for antibodies against double-stranded DNA (dsDNA) for diagnostics of chronic autoimmune diseases, such as systemic lupus erythematosus (SLE). The fluorometric immunoassay method for detection of anti-dsDNA solves the technical problem of designing a method for detection of the aforesaid antibodies, which would be faster, cheaper and less toxic as the standard Farr-RIA method, but would have the same diagnostic specificity (which is 100%) and improved diagnostic sensitivity (for 3%). Detection of anti-dsDNA is based on detection of fluorescence in two fractions of samples, in the supernatant and in the sample with a precipitate, which contains immune complexes composed of added dsDNA and anti-dsDNA present in the patient's serum, wherein the detected fluorescence is a consequence of binding fluorescent dyes with dsDNA bound in the complexes or with free dsDNA. The method according to the invention allows obtaining reliable results, fast sample analysis and results availability to the attending physician, use of human and environmentally safe chemicals and lowering of costs in comparison to the standard Farr-RIA method.
Keywords		fluorometrična imunska metoda diagnostika avtoimunskih bolezni določanje anti-dsDNA