Author/Editor		Phuong, Tam T. T.; Yarishkin, Oleg; Križaj, David
Title		Subcellular propagation of calcium waves in Müller glia does not require autocrine/paracrine purinergic signaling
Type		članek
Vol. and No.		Letnik 10, št. 5
Publication year		2016
Volume		str. 421-427
ISSN		1933-6950 - Channels (Austin, Tex.)
Language		eng
Abstract		The polarized morphology of radial glia allows them to functionally interconnect different layers of CNS tissues including the retina, cerebellum, and cortex. A likely mechanism involves propagation of transcellular Ca 2 C waves which were proposed to involve purinergic signaling. Because it is not known whether ATP release is required for astroglial Ca 2 C wave propagation we investigated this in mouse Müller cells, radial astroglia-like retinal cells in which in which waves can be induced and supported by Orai/TRPC1 (transient receptor potential isoform 1) channels. We found that depletion of endoplasmic reticulum (ER) stores triggers regenerative propagation of transcellular Ca 2 C waves that is independent of ATP release and activation of P 2 X and P 2 Y receptors. Both the amplitude and kinetics of transcellular, depletion-induced waves were resistant to non-selective purinergic P 2 antagonists such as pyridoxalphosphate-6-azophenyl-2 0 ,4 0 -disulfonic acid (PPADS). Thus, store- operated calcium entry (SOCE) is itself sufficient for the initiation and subcellular propagation of calcium waves in radial glia.
Keywords		calcium entry subcellular propagation cerebellum vnos kalcija subcelično razmnoževanje mali možgani