| Author/Editor | Srebotnik-Kirbiš, Irena; Strojan Fležar, Margareta | |
| Title | Cell count-based triaging of cytology samples for cell block preparation | |
| Type | članek | |
| Publication year | 2016 | |
| Volume | str. str. | |
| ISSN | 0956-5507 - Cytopathology : official journal of the British Society for Clinical Cytology | |
| Language | eng | |
| Abstract | Objective: Cytology samples can sometimes yield cell blocks of low cellularity that are inadequate for cyto- morphological evaluation and ancillary studies. The purpose of this study was to analyse the value of cell count based-triaging of samples for cell block preparation. Methods: Cell blocks were prepared from different cytology samples using HistoGel TM according to the man- ufacturer's instructions following cell count assessment. The adequacy of cell blocks was semi-quantitatively scored as empty, scanty, moderate or highly cellular by evaluating corresponding haematoxylin and eosin (H&E) sections. Cell blocks with moderate and highly cellular H&E sections were considered adequate whereas cell blocks with empty and scanty cells in H&E sections were inadequate for cytomorphological eval- uation. The average, range and a median number of cells ( x 10[sup]6 ) embedded in adequate and inadequate cell blocks were compared. Results: Among 54 cell blocks prepared from fine needle aspiration biopsies (FNAs, 32), effusions (4), brush- ings (13) and cell lines (5), 20 cell blocks (37%) yielded moderate or highly cellular H&E sections and were considered adequate whereas 34 (63%) cell blocks were empty or scanty and thus considered inadequate. The average ( +- SD) number of cells ( X 10[sup]6 ) embedded in adequate and inadequate cell blocks was 2.1 +- 4.3 and 0.5 +- 0.3, respectively. Samples containing at least 1 x 10[sup]6 cells yielded adequate cell blocks in 12/16 cases (75%) whereas samples with less than 1 X 10[sup]6 cells in 13/38 (34%) cases. Conclusion: The present study showed that cell count-based triaging of cytology samples could reduce the number of low cellular cell blocks. | |
| Keywords | citologija vzorci število celic cytology samples cell count |