Author/Editor		Shao, Wei; Žumer, Kristina; Fujinaga, Koh; Peterlin, Matija Boris
Title		FBXO3 protein promotes ubiquitylation and transcriptional activity of AIRE (autoimmune regulator)
Type		članek
Vol. and No.		Letnik 291, št. 34
Publication year		2016
Volume		str. 17953-17963
ISSN		0021-9258 - The Journal of biological chemistry
Language		eng
Abstract		The autoimmune regulator (AIRE) is a transcription factor which is expressed in medullary thymic epithelial cells. It directs the expression of otherwise tissue-specific antigens, which leads to the elimination of autoreactive T cells during development. AIRE is modified post-translationally by phosphorylation and ubiquitylation. In this report we connected these modifications. AIRE, which is phosphorylated on two specific residues near its N terminus, then binds to the F-box protein 3 (FBXO3) E3 ubiq- uitin ligase. In turn, this SCF FBXO3 (SKP1-CUL1-F box) complex ubiquitylates AIRE, increases its binding to the positive tran- scription elongation factor b (P-TEFb), and potentiates its transcriptional activity. Because P-TEFb is required for the transition from initiation to elongation of transcription, this interaction ensures proper expression of AIRE-responsive tissue-specific antigens in the thymus.
Keywords		epithelial cells transcription factor autoimmune regulator epitelijske celice transkripcijski faktor autoimunski regulator