Author/Editor		Blazek, Dalibor; Teque, Fernando; Mackewicz, Carl; Peterlin, Matija Boris; Levy, Jay A.
Title		The CD8+ cell non-cytotoxic antiviral response affects RNA polymerase II-mediated human immunodeficiency virus transcription in infected CD4+ cells
Type		članek
Vol. and No.		Letnik 97, št. 1
Publication year		2016
Volume		str. 220-224
ISSN		0022-1317 - The Journal of general virology
Language		eng
Abstract		A CD8+ cell non-cytotoxic antiviral response (CNAR), mediated by a CD8+ cell antiviral factor (CAF), is associated with a long-term healthy state in human immunodeficiency virus (HIV) infection. CNAR/CAF reduces viral transcription without a known effect on specific viral sequences in the HIV genome. In studies to define the mechanism involved in the block in viral transcription, we now report that transcription from the HIV-LTR reporter is reduced in infected CD4+ cells upon treatment with CAF. In agreement with this observation, the amount of RNA polymerase II (RNAPII) on the HIV promoter and other viral regions was strongly diminished in HIV-infected CD4+ cells co-cultivated with CNAR-expressing CD8+ cells. These results demonstrate further that CNAR/CAF has a specific role in regulating HIV transcription and a step during the preinitiation complex assembly appears to be sensitive to CNAR/CAF.
Keywords		immunodeficiency CD8+ cells antiviral response imunska pomanjkljivost CD8+ celice protivirusni odziv