| Author/Editor | Dumke, Roger; Benitez, Alvaro J.; Chalker, Victoria J.; Gullsby, Karolina; Henrich, Birgit; Hidalgo-Grass, Carlos; Hoogenboezem, Theo; Keše, Darja; Loens, Katherine; Maaskant, Jolanda | |
| Title | Multi-center evaluation of one commercial and 12 in-house real-time PCR assays for detection of Mycoplasma pneumoniae | |
| Type | članek | |
| Vol. and No. | Letnik 88, št. 2 | |
| Publication year | 2017 | |
| Volume | str. 111-114 | |
| ISSN | 1879-0070 - Diagnostic microbiology and infectious disease | |
| Language | eng | |
| Abstract | Detection of Mycoplasma pneumoniae by real-time PCR is not yet standardized across laboratories.We have implemented a standardization protocol to compare the performance of thirteen commercial and in-house approaches. Despite differences on threshold values of samples, all assays were able to detect at least 20 M. pneumoniae genomes per reaction. | |
| Keywords | Mycoplasma pneumoniae detection real-time PCR Mycoplasma pneumoniae odkrivanje PCR v realnem času |