Author/Editor | Rozman, Jasmina Živa; Pohar Perme, Maja; Jež, Mojca; Maličev, Elvira; Krašna, Metka; Vrtovec, Bojan; Rožman, Primož | |
Title | DNA methylation and hydroxymethylation Profile of CD34+-enriched cell products intended for autologous CD34+ cell transplantation | |
Type | članek | |
Publication year | 2017 | |
Volume | str. str. | |
ISSN | 1044-5498 - DNA and cell biology | |
Language | eng | |
Abstract | Epigenetic dysregulation has been shown to limit functional capacity of aging hematopoietic stem cells, which may contribute to impaired outcome of hematopoietic stem cell-based therapies. The aim of our study was to gain better insight into the epigenetic profile of CD34+-enriched cell products intended for autologous CD34+ cell transplantation in patients with cardiomyopathy. We found global DNA methylation content significantly higher in immunoselected CD34+ cells compared to leukocytes in leukapheresis products (2.33 +- 1.03% vs. 1.84 +- 0.86%, p=0.04). Global DNA hydroxymethylation content did not differ between CD34+ cells and leukocytes (p=0.30). By measuring methylation levels of 94 stem cell transcription factors on a ready-to-use array, we identified 15 factors in which average promoter methylation was significantly different between leukocytes and CD34+ cells. The difference was highest for HOXC12 (58.18 +- 6.47% vs. 13.34 +- 24.18%, p=0.0009) and NR2F2 (51.65 +-25.89% vs. 7.66 +- 21.43%, p=0.0045) genes. Our findings suggest that global DNA methylation and hydroxymethylation patterns as well as target methylation profile of selected genes in CD34+-enriched cell products do not differ significantly compared to leukapheresis products and, thus, can tell us little about the functional capacity and regenerative properties of CD34+ cells. Future studies should examine other CD34+ cell graft characteristics, which may serve as prognostic tools for autologous CD34+ cell transplantation. | |
Keywords | epigenetics heart failure DNA methylation epigenetika odpoved srca metilacija DNA |