Author/Editor     Čemažar, Maja; Ambrožič, Jerneja; Pavlin, Darja; Serša, Gregor; Poli, Alessandro; Krhač Levačić, Ana; Tešić, Nataša; Lampreht Tratar, Urša; Rak, Mitja; Tozon, Nataša
Title     Efficacy and safety of electrochemotherapy combined with peritumoral IL-12 gene electrotransfer of canine mast cell tumours
Type     članek
Vol. and No.     Letnik 15, št. 2
Publication year     2017
Volume     str. 641-654
ISSN     1476-5829 - Veterinary and comparative oncology
Language     eng
Abstract     Electrochemotherapy combined with peritumoral interleukin-12 (IL-12) gene electrotransfer was used for treatment of mast cell tumours in 18 client-owned dogs. Local tumour control, recurrence rate, as well as safety of combined therapy were evaluated. One month after the therapy, no side effects were recorded and good local tumour control was observed with high complete responses rate which even increased during the observation period to 72%. IL-12 gene electrotransfer resulted in 78% of patients with detectable serum IFN-% and/or IL-12 levels. In the treated tumours vascular changes as well as minimal T-lymphocytes infiltration was observed. After 1 week, the plasmid DNA was not detected intra- or peritumorally and no horizontal gene transfer was observed. In summary, our study demonstrates high antitumour efficacy of electrochemotherapy combined with IL-12 electrotransfer, which also prevented recurrences or distant metastases, as well as its safety and feasibility in treatment of canine mast cell tumours.
Keywords     dogs
efficacy
electrochemotherapy
interleukin-12
psi
učinkovitost
elektrokemoterapija
interlevkin-12