Author/Editor     Matjašič, Alenka; Tajnik, Mojca; Boštjančič, Emanuela; Popović, Mara; Matos, Boštjan; Glavač, Damjan
Title     Identifying novel glioma-associated noncoding RNAs by their expression profiles
Type     članek
Vol. and No.     , št. Vol. 2017
Publication year     2017
Volume     str. 1-18
ISSN     2314-436X - International journal of genomics
Language     eng
Abstract     Long noncoding RNAs (lncRNAs) and microRNAs (miRNAs) play a significant role in cancer development as regulators of protein-coding genes. Their dysregulation was in some extent already associated with glioma, the most aggressive primary brain tumours in adults. The correct diagnosis and treatment selection due to high tumour heterogeneity might be difficult and inadequate, resulting in poor prognosis. Studies of expression patterns of noncoding RNAs (ncRNAs) could provide useful insight in glioma molecular development. We used the qPCR approach to screen and investigate the expression of lncRNAs that were previously deregulated in other cancer types. The study showed altered expression levels for numerous lncRNAs across histologically different glioma samples. Validation of few lncRNAs showed association of expression levels with histological subtype and/or malignancy grade. We also observed deregulated and subtype-distinctive expression for four lncRNA-associated miRNAs. Expression of few lncRNAs and miRNA was also associated with patients% survival, showing potential prognostic value. Several ncRNAs, some already related to glioma and some, to the best of our knowledge, investigated for the first time, might be of greater importance in glioma molecular development and progression. Finding the subtype-specific lncRNA and/or miRNA expression patterns may contribute additional information for a more objective classification.
Keywords     nekodirajoči RNA
gliom
možgani
noncoding RNAs
gliom
brain