| Author/Editor | Lakota, Katja; Hrušovar, Dolores; Ogrič, Manca; Mrak Poljšak, Katjuša; Čučnik, Saša; Tomšič, Matija; Božič, Borut; Žigon, Polona; Sodin-Šemrl, Snežna | |
| Title | Analysis of drug effects on primary human coronary artery endothelial cells activated by serum amyloid A | |
| Type | članek | |
| Vol. and No. | , št. Vol. 2018 | |
| Publication year | 2018 | |
| Volume | str. 1-11 | |
| ISSN | 0962-9351 - Mediators of inflammation | |
| Language | eng | |
| Abstract | Background. RA patients have a higher incidence of cardiovascular diseases compared to the general population. Serum amyloid A (SAA) is an acute-phase protein, upregulated in sera of RA patients. Aim. To determine the effects of medications on SAA-stimulated human coronary artery endothelial cells (HCAEC). Methods. HCAEC were preincubated for 2%h with medications from sterile ampules (dexamethasone, methotrexate, certolizumab pegol, and etanercept), dissolved in medium (captopril) or DMSO (etoricoxib, rosiglitazone, meloxicam, fluvastatin, and diclofenac). Human recombinant apo-SAA was used to stimulate HCAEC at a final 1000%nM concentration for 24%hours. IL-6, IL-8, sVCAM-1, and PAI-1 were measured by ELISA. The number of viable cells was determined colorimetrically. Results. SAA-stimulated levels of released IL-6, IL-8, and sVCAM-1 from HCAEC were significantly attenuated by methotrexate, fluvastatin, and etoricoxib. Both certolizumab pegol and etanercept significantly decreased PAI-1 by an average of 43%. Rosiglitazone significantly inhibited sVCAM-1 by 58%. Conclusion. We observed marked influence of fluvastatin on lowering cytokine production in SAA-activated HCAEC. Methotrexate showed strong beneficial effects for lowering released Il-6, IL-8, and sVCAM-1. Interesting duality was observed for NSAIDs, with meloxicam exhibiting opposite-trend effects from diclofenac and etoricoxib. This represents unique insight into specific responsiveness of inflammatory-driven HCAEC relevant to atherosclerosis. | |
| Keywords | drug effects coronary artery serum amyloid učinki zdravil koronarna arterija serumski amiloid |