Author/Editor		Zupanec, Neja; Jovchevska, Ivana; Stojan, Jure; Zottel, Alja; Liović, Mirjana; Myers, Michael P.; Muyldermans, Serge; Šribar, Jernej; Križaj, Igor; Komel, Radovan
Title		Glioblastoma-specific anti-TUFM nanobody for in-vitro immunoimaging and cancer stem cell targeting
Type		članek
Vol. and No.		Letnik 9, št. 25
Publication year		2018
Volume		str. 17282-17299
ISSN		1949-2553 - Oncotarget
Language		eng
Abstract		Glioblastoma multiforme (GBM) is the most common and lethal form of brain tumor. The prognosis for patients remains poor, despite the combination of new preoperative and intraoperative neuroimaging, radical surgery, and recent advances in radiotherapy and chemotherapy. To improve GBM therapy and patient outcome, sustained drug delivery to glioma cells is needed, while minimizing toxicity to adjacent neurons and glia cells. This might be achieved through an anti-proteomic approach based on nanobodies, the single-domain antigen-binding fragments of heavy-chain antibodies of the camelid adaptive immune system. We report here on the validation and quantification of a nanobody raised against mitochondrial translation elongation factor (TUFM). Differential expression of TUFM was examined in different GBM cell lines and GBM tissue at the protein and mRNA levels, as compared to their expression in neural stem cells and normal brain tissue. We further used in-silico modelling and immunocytochemistry to define the specificity of anti-TUFM nanobody (Nb206) towards GBM stem cells, as compared to GBM cell lines (U251MG and U87MG cells). Due to its specificity and pronounced inhibitory effect on GBM stem cell growth, we propose the use of this anti-TUFM nanobody for GBM in vitro immunoimaging and potentially also cancer stem cell targeting.
Keywords		glioblastoma multiforme biomarkers nanobodies multiformni glioblastom označevalci nanotelesa