Author/Editor | Rupnik, M; Law, GJ; Northrop, AJ; Mason, WT; Zorec, R | |
Title | Brefeldin A and a synthetic peptide to ADP-ribosylation factor (ARF) inhibit regulated exocytosis in melanotrophs | |
Type | članek | |
Source | Neuroreport | |
Vol. and No. | Letnik 6, št. 6 | |
Publication year | 1995 | |
Volume | str. 853-6 | |
Language | eng | |
Abstract | We investigated the role of ADP-ribosylation factor (ARF) in regulated exocytosis in patch-clamped rat melanotrophs. Addition of brefeldin A (BFA) to inhibit activation of endogenous ARF protein was found to attenuate regulated secretory activity monitored as changes in membrane capacitance (Cm). A synthetic peptide to amino acids 46-61 of ARF (P-14) was also found to inhibit Ca(2+)-induced secretory activity in these cells. This inhibition was not apparent with a scrambled amino acid sequence of ARF-P14 peptide. This paper provides the first patch-clamp study to suggest that the small GTP-binding protein ARF is required to trigger release of secretory granules from rat pituitary melanotrophs. | |
Descriptors | ADENYL CYCLASE CYCLOPENTANES EXOCYTOSIS G-PROTEINS PITUITARY GLAND, ANTERIOR PROTEIN SYNTHESIS INHIBITORS AMINO ACID SEQUENCE CALCIUM CHROMATOGRAPHY, HIGH PRESSURE LIQUID MOLECULAR SEQUENCE DATA PATCH-CLAMP TECHNIQUES PITUITARY GLAND, ANTERIOR RATS |