Author/Editor		Yarishkin, Oleg; Phuong, Tam T. T.; Lakk, Monika; Križaj, David
Title		TRPV4 does not regulate the distal retinal light response
Type		članek
Source		In: Retinal degenerative diseases : mechanisms and experimental therapy [S. l.] : Springer International Publishing.
Publication year		2018
Volume		str. 553-560
ISSN		2214-8019 - Advances in experimental medicine and biology
Language		eng
Abstract		The transient receptor potential vanilloid isoform 4 (TRPV4) functions as polymodal transducer of swelling, heat, stretch, and lipid metabolites, is widely expressed across sensory tissues, and has been implicated in pressure sensing in vertebrate retinas. Although TRPV4 knockout mice exhibit a variety of mechanosensory, nociceptive, and thermo- and osmoregulatory phenotypes, it is not known whether the transmission of light-induced signals in the eye is affected by the loss of TRPV4. We utilized field potentials, a measure of rod and cone signaling, to determine whether TRPV4 impacts on the generation and/or transmission of the photoreceptor light response and neurotransmission. Luminance intensity-response relationships were acquired in anesthetized wild-type and TRPV4%/% mice and evaluated for peak amplitude and implicit time under scotopic and photopic conditions. We found that the morphology of the outer retina is unaffected by the ablation of the Trpv4 gene. Calcium imaging of dissociated Müller glia showed that selective TRPV4 stimulation induces oscillatory calcium signals in adjacent rods. However, no differences in scotopic or photopic light-evoked signaling in the distal retina were observed in TRPV4-/- eyes, suggesting that TRPV4 signaling in healthy Müller cells does not modulate the transmission of light-evoked signals at rod and cone synapses.
Keywords		retina TRPV4 photoreceptor mrežnica TRPV4 fotoreceptor