Author/Editor		Lopes, Alessandra; Vanvarenberg, Kevin; Kos, Špela; Lucas, Sophie; Colau, Didier; Van den Eynde, Benoît; Préat, Véronique; Vandermeulen, Gaëlle
Title		Combination of immune checkpoint blockade with DNA cancer vaccine induces potent antitumor immunity against P815 mastocytoma
Type		članek
Vol. and No.		Letnik 8, št. 1
Publication year		2018
Volume		str. 1-11
ISSN		2045-2322 - Scientific reports
Language		eng
Abstract		DNA vaccination against cancer has become a promising strategy for inducing a specific and long-lasting antitumor immunity. However, DNA vaccines fail to generate potent immune responses when used as a single therapy. To enhance their activity into the tumor, a DNA vaccine against murine P815 mastocytoma was combined with antibodies directed against the immune checkpoints CTLA4 and PD1. The combination of these two strategies delayed tumor growth and enhanced specific antitumor immune cell infiltration in comparison to the corresponding single therapies. The combination also promoted IFNg, IL12 and granzyme B production in the tumor microenvironment and decreased the formation of liver metastasis in a very early phase of tumor development, enabling 90% survival. These results underline the complementarity of DNA vaccination and immune checkpoint blockers in inducing a potent immune response, by exploiting the generation of antigen-specific T cells by the vaccine and the ability of immune checkpoint blockers to enhance T cell activity and infiltration in the tumor. These findings suggest how and why a rational combination therapy can overcome the limits of DNA vaccination but could also allow responses to immune checkpoint blockers in a larger proportion of subjects.
Keywords		eksperimentalna onkologija zaviralci imunskih stikal imunoterapija DNK cepivo proti raku eksperimental oncology immune checkpoint blocade DNA cancer vaccine immunotherapy