Author/Editor		Žarković, Kamelija; Uchida, Koji; Kolenc, Danijela; Hlupic, Ljiljana; Žarković, Neven
Title		Tissue distribution of lipid peroxidation product acrolein in human colon carcinogenesis
Type		članek
Vol. and No.		Letnik 40, št. 6
Publication year		2006
Volume		str. 543-552
ISSN		1071-5762 - Free radical research : official journal of the Society for Free Radical Research - European Region and the Society for Free Radical Research - Asia
Language		eng
Abstract		Lipid peroxidation product acrolein, well-known pollutant in tobacco and automotive smoke, accumulatesin vivobound toproteins. It suppresses p53 synthesis acting as potent carcinogenic factor for oral, respiratory and bladder carcinomas, while itspossible association with colon carcinogenesis was not studied so far. We used genuine monoclonal antibody to evaluateimmunohistochemical distribution of acrolein - protein adducts in 113 human colon tumours. The presence of acrolein - protein adducts was increasing with respect to colon carcinogenesis, from moderate appearance in tubular and villotubularlow-grade adenomas to abundant and diffuse distribution in high-grade villotubular adenomas and Dukes A carcinomas.However, in advanced Dukes B and C carcinomas acrolein was hardly noticed, although, its protein adducts were foundabundant in non-malignant colon epithelium of these patients. There was no relationship between p53 and acroleindistribution. According to these findings, acrolein seems to be lipid peroxidation product associated with transition frombenign into malignant colon tumours.
Keywords		lipid peroxidation colon carcinogenesis oxidative stress lipidna peroksidacija oksidativni stres karcinogeneza kolona