Author/Editor		Bates, Russell; Irving, Benjamin; Markelc, Boštjan; Kaeppler, Jakob; Brown, Graham; Muschel, Ruth J.; Brady, J. Michael; Grau, Vincente; Schnabel, Julia A.
Title		Segmentation of vasculature from fluorescently labeled endothelial cells in multi-photon microscopy images
Type		članek
Vol. and No.		Letnik 38, št. 1
Publication year		2019
Volume		str. 1-10
ISSN		0278-0062 - IEEE transactions on medical imaging
Language		eng
Abstract		Vasculature is known to be of key biological significance, especially in the study of tumors. As such, considerable effort has been focused on the automated segmentation of vasculature in medical and pre-clinical images. The majority of vascular segmentation methods focus on bloodpool labeling methods, however, particularly in the study of tumors it is of particular interest to be able to visualize both perfused and non-perfused vasculature. Imaging vasculature by highlighting the endothelium provides a way to separate the morphology of vasculature from the potentially confounding factor of perfusion. Here we present a method for the segmentation of tumor vasculature in 3D fluorescence microscopy images using signals from the endothelial and surrounding cells. We show that our method can provide complete and semantically meaningful segmentations of complex vasculature using a supervoxel-Markov Random Field approach. We show that in terms of extracting meaningful segmentations of the vasculature, our method out-performs both a state-ofthe- art method, specific to these data, as well as more classical vasculature segmentation methods.
Keywords		ožilje segmentacija endotelijske celice krvne preiskave vasculature segmentation endothelial cells bloodpool labeling methods