Author/Editor		Stojan, Jure
Title		Rapid mechanistic evaluation and parameter estimation of putative inhibitors in a single-step progress-curve analysis
Type		članek
Vol. and No.		Letnik 22, št. 8
Publication year		2017
Volume		str. 1-9
ISSN		1420-3049 - Molecules (Basel, Switzerland)
Language		eng
Abstract		Highly efficient and rapid lead compound evaluation for estimation of inhibition parameters and type of inhibition is proposed. This is based on a single progress-curve measurement in the presence of each candidate compound, followed by the simultaneous analysis of all of these curves using the ENZO enzyme kinetics suite, which can be implemented as a web application. In the first step, all of the candidate ligands are tested as competitive inhibitors. Where the theoretical curves do not correspond to the experimental data, minimal additional measurements are added, with subsequent processing according to modified reaction mechanisms.
Keywords		enzymic mechanism inhibitors reactivators encimski mehanizem inhibitorji reaktivatorji