Author/Editor		Dolinar, Ana; Koritnik, Blaž; Glavač, Damjan; Ravnik-Glavač, Metka
Title		Circular RNAs as potential blood biomarkers in amyotrophic lateral sclerosis
Type		članek
Publication year		2019
Volume		str. str.
ISSN		0893-7648 - Molecular neurobiology
Language		eng
Abstract		Circular RNAs (circRNAs) are emerging as a novel, yet powerful player in many human diseases. They are involved in severalcellular processes and are becoming a noteworthy type of biomarkers. Among other functions, circRNAs can serve as RNAsponges or as scaffolds for RNA-binding proteins. Here, we investigated a microarray expression profile of circRNAs inleukocyte samples from ALS patients and age- and sex-matched healthy controls to identify differentially expressedcircRNAs. We selected 10 of them for a qPCR validation of expression on a larger set of samples, identification of theirassociations with clinical parameters, and evaluation of their diagnostic potential. In total, expression of 7/10 circRNAs wassignificant in a larger cohort of ALS patients, compared with age- and sex-matched healthy controls. Three of them(hsa_circ_0023919, hsa_circ_0063411, and hsa_circ_0088036) showed the same regulation as in microarray results. These threecircRNAs also had AUC > 0.95, and sensitivity and specificity for the optimal threshold point > 90%, showing their potential forusing them as diagnostic biomarkers.
Keywords		amiotrofična lateralna skleroza biomarkerji vzorci človeške krvi amyotrophic lateral sclerosis biomarkers human blood samples