Author/Editor		Resnik, Nataša; Erman, Andreja; Veranič, Peter; Erdani-Kreft, Mateja
Title		Triple labelling of actin filaments, intermediate filaments and microtubules for broad application in cell biology
Type		članek
Publication year		2019
Volume		str. str.
ISSN		0948-6143 - Histochemistry and cell biology
Language		eng
Abstract		We report a protocol for simultaneous triple labelling of intermediate filaments, microtubules and actin filaments. The described procedure offers an optimal preservation of the structure and antigenicity of individual representatives of cytoskeletal elements and is applicable for labelling of tissue samples and cultured cells. Namely, we demonstrate that using this protocol the cytoskeletal elements are well-preserved and detectable in the whole mount urinary bladder tissue pieces, cryosections of the urinary bladder, and in cultured normal and cancer urothelial cells including their delicate intercellular connections such as tunneling nanotubes (TnTs). The protocol uncovers for the first time the co-distribution of actin filaments, intermediate filaments and microtubules in TnTs, which were up to now known as mono- or bi-cytoskeletal structures. Presented triple labelling protocol provides an efficient tool for studying co-distribution of actin filaments, intermediate filaments, and microtubules and therefore offers new insights into their cellular and tissue distribution.
Keywords		urothelial cells actin filaments microtubules urotelijske celice aktinski filamenti mikrotubuli