Author/Editor     Zajc, Irena
Title     Mikrosateliti in testiranje starševstva pri govedu
Translated title     Microsatellites and paternity testing in cattle
Type     članek
Source     Zb Vet Fak Univ Ljublj
Vol. and No.     Letnik 32, št. 2
Publication year     1995
Volume     str. 217-26
Language     slo
Abstract     In the paper, it was demonstrated that the published bovine microsatellites may be used to perorm accurate paternity and maternity test in cattle. They offer an intersting and relatively easily analysed system for comparing the DNA of offspring with DNA extracted from their suspected parents. Usually, as little as one-tenth of a millilitre of blood is sufficent to provide the DNA needed for the test. Ideally, the blood should not be allowed to clot, but we have developed procedures for isolating the DNA from clotted blood samples that were several years old, provided that they had been properly frozen. Presently, the techique will only allow the definite exclusion of individuals that were not parents. The successful outcome of a test therefore requires analysing all potential mothers and fathers in order that the true parents be identified by a process of elimination. Parentage testing in domestic animals is mainly limited to paternity testing. Because of the codominant nature of microsatellites and their strictly Mendelian fashion of inheritance, there is no reason why they should not be equally used for maternity testings as well as to study the relathionships within closely related, highly inbred groups of domestic animals. The utility of microsatellites in such studies has been demonstrated by the present rearch on relationships whitin the inbred group of cattle of a single breeder; the parentage of all the calves in the group tested has been successfuly and accurately assigned.
Descriptors     GENOMIC IMPRINTING
DNA, SATELLITE
CATTLE
POLYMERASE CHAIN REACTION