Author/Editor     Lindtner, R
Title     Piščanci v diagnostiki leptospiroze
Translated title     Baby chicks in the diagnosis of leptospirosis
Type     članek
Source     Zb Vet Fak Univ Ljublj
Vol. and No.     Letnik 32, št. 1
Publication year     1995
Volume     str. 83-99
Language     slo
Abstract     The studies for detecting the suitability of baby chicks for the diagnosis of leptospirosis were conducted on one-day old and six-days old chickens of SPF flock, of Nick Chick origin. Preliminary experiments with the laboratory strains of the serovars icterohaemorrhagiae, pomona, grippothyphosa and australis on one day old chickens confirmed the variable susceptibility of chickens for various strains of leptospires. The suitability of one-day old chickens for inoculation of the applied strains of laboratory cultivated leptospires was established. Pathogen strains of the serovars icterohaemorrhagiae (human isolate) and pomona (swine isolate) were used in the main research. One-day old chickens and six-days old chickens were inoculated with the isolated pathogen strains. Direct bacteriological cultivation for reisolation of the inoculated strains from blood, liver and renal tissues of the infected animals was performed. Blood samples were tested in the microagglutination test to detect antibodies. Histological examinations and the Levaditi silver impregnation test showed leptospires in tissues and severe changes in the internal organs of the infected animals. We established one-day old chickens to be very useful for the diagnosis of leptospirosis. Six-days old chickens were less susceptible. In our experiment of applying the pathogenic material on one-day old chickens, blood and emulsion of liver and kidney of infected golden hamsters were used. Successful cultivation, serological and histological tests of infected one-day old chickens showed their suitability for the isolati on of leptospires from pathogenic material. We established that the best time for cultivation of inoculated leptospires from the blood of infected chickens was seven days after inoculation. Reisolation of the inculum from their live and renal tissues was most effective 14 days after inoculation. Serological tests should be made from 14 to 21 days after inoculation of leptospires.(Abstract trunca
Descriptors     ANIMAL DISEASES
LEPTOSPIROSIS
CHICKENS
ANIMALS, DOMESTIC