| Author/Editor | Reusken, Chantal; Boonstra, Marrit; Rugebregt, Sharona; Scherbeijn, Sandra; Chandler, Felicity; Avšič-Županc, Tatjana; Vapalahti, Olli; Koopmans, Marion; Geurtsvan Kessel, Corine H. | |
| Title | An evaluation of serological methods to diagnose tick-borne encephalitis from serum and cerebrospinal fluid | |
| Type | članek | |
| Vol. and No. | , št. Vol. 120 | |
| Publication year | 2019 | |
| Volume | str. 78-83 | |
| ISSN | 1386-6532 - Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology | |
| Language | eng | |
| Abstract | Background:Tick-borne encephalitis (TBE) is an infectious disease endemic to large parts of Europe and Asia.Diagnosing TBE often relies on the detection of TBEV-specific antibodies in serum and cerebrospinalfluid (CSF)as viral genome is mostly not detectable once neurological symptoms occur.Objectives:We evaluated the performance of TBEV IgM and IgG ELISAs in both serum and CSF of confirmedTBEV patients and discuss the role of (CSF) serology in TBEV diagnostics.Study design:For the assay evaluation we collected specimen from confirmed TBEV patients. Assay specificitywas assessed using sera from patients with a relatedflavivirus infection or other acute infection. A selected ELISAassay was used to analyze TBEV-specific antibodies in CSF and to evaluate the use in confirming TBE diagnosis.Results:In this study the overall sensitivity of the IgM TBEV ELISAs was acceptable (94 -100 %). Four out offiveIgM ELISA%s demonstrated an excellent overall specificity from 94 -100% whereas a low overall specificity wasobserved for the IgG TBEV ELISAs (30-71%). Intrathecal antibody production against TBEV was demonstrated ina subset of TBE patients.Conclusions:In four out offive ELISAs, IgM testing in serum and CSF of TBE patients is specific and confirmative.The lack of IgG specificity in all ELISAs emphasizes the need of confirmatory testing by virus neutralisation,depending on the patient%s background and the geographic location of exposure to TBEV. A CSF-serum IgGantibody index can support the diagnosis specifically in chronic disease or once IgM has disappeared. | |
| Keywords | virus encefalitisa, ki se prenaša s klopi serologija ELISA tick-borne encephalitis virus serology ELISA |